Genetically-targeted photorelease of endocannabinoids enables optical control of GPR55 in pancreatic β-cells.

ABSTRACT: Fatty acid amides (FAAs) are a family of second-messenger lipids that target cannabinoid receptors, and are known mediators of glucose-stimulated insulin secretion from pancreatic β-cells. Due to the diversity observed in FAA structure and pharmacology, coupled with the expression of at least 3 different cannabinoid G protein-coupled receptors in primary and model β-cells, our understanding of their role is limited by our inability to control their actions in time and space. To investigate the mechanisms by which FAAs regulate β-cell excitability, we developed the Optically-Cleavable Targeted (OCT)-ligand approach, which combines the spatial resolution of self-labeling protein (SNAP-) tags with the temporal control of photocaged ligands. By linking a photocaged FAA to an o-benzylgua

SUBMITTER: Tobias JM 

PROVIDER: S-EPMC8528030 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature