PIR-B Regulates CD4⁺ IL17a⁺ T-Cell Survival and Restricts T-Cell-Dependent Intestinal Inflammatory Responses.

ABSTRACT:

Background & aims

CD4⁺ T cells are regulated by activating and inhibitory cues, and dysregulation of these proper regulatory inputs predisposes these cells to aberrant inflammation and exacerbation of disease. We investigated the role of the inhibitory receptor paired immunoglobulin-like receptor B (PIR-B) in the regulation of the CD4⁺ T-cell inflammatory response and exacerbation of the colitic phenotype.

Methods

We used Il10^-/- spontaneous and CD4⁺CD45RB^hi T-cell transfer models of colitis with PIR-B-deficient (Pirb^-/-) mice. Flow cytometry, Western blot, and RNA sequencing analysis was performed on wild-type and Pirb^-/- CD4⁺ T cells. In silico analyses were performed on RNA sequencing

SUBMITTER: Uddin J 

PROVIDER: S-EPMC8531983 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature