Dataset Information

Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the Stabilitome.

ABSTRACT: The thermal stabilities of endogenous, intact proteins and protein assemblies in complex mixtures were characterized in parallel by means of variable-temperature electrospray ionization coupled to mass spectrometry (vT-ESI-MS). The method is demonstrated by directly measuring the melting transitions of seven proteins from a mixture of proteins derived from ribosomes. A proof-of-concept measurement of a fraction of an Escherichia coli lysate is provided to extend this approach to characterize the thermal stability of a proteome. As the solution temperature is increased, proteins and protein complexes undergo structural and organizational transitions; for each species, the folded ↔ unfolded and assembled ↔ disassembled populations are monitored based on changes in vT-ESI-MS charge state distributions and masses. The robustness of the approach illustrates a step toward the proteome-wide characterization of thermal stabilities and structural transitions-the stabilitome.

SUBMITTER: El-Baba TJ

PROVIDER: S-EPMC8546744 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the <i>Stabilitome</i>.

El-Baba Tarick J TJ Raab Shannon A SA Buckley Rachel P RP Brown Christopher J CJ Lutomski Corinne A CA Henderson Lucas W LW Woodall Daniel W DW Shen Jiangchuan J Trinidad Jonathan C JC Niu Hengyao H Jarrold Martin F MF Russell David H DH Laganowsky Arthur A Clemmer David E DE

Analytical chemistry 20210608 24

The thermal stabilities of endogenous, intact proteins and protein assemblies in complex mixtures were characterized in parallel by means of variable-temperature electrospray ionization coupled to mass spectrometry (vT-ESI-MS). The method is demonstrated by directly measuring the melting transitions of seven proteins from a mixture of proteins derived from ribosomes. A proof-of-concept measurement of a fraction of an <i>Escherichia coli</i> lysate is provided to extend this approach to character ...[more]

PMID: 34101419

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Dataset Information

Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the Stabilitome.

Publications

Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the <i>Stabilitome</i>.

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