Taiji-reprogram: a framework to uncover cell-type specific regulators and predict cellular reprogramming cocktails.

ABSTRACT: Cellular reprogramming is a promising technology to develop disease models and cell-based therapies. Identification of the key regulators defining the cell type specificity is pivotal to devising reprogramming cocktails for successful cell conversion but remains a great challenge. Here, we present a systems biology approach called Taiji-reprogram to efficiently uncover transcription factor (TF) combinations for conversion between 154 diverse cell types or tissues. This method integrates the transcriptomic and epigenomic data to construct cell-type specific genetic networks and assess the global importance of TFs in the network. Comparative analysis across cell types revealed TFs that are specifically important in a particular cell type and often tightly associated with cell-type specific f

SUBMITTER: Wang J 

PROVIDER: S-EPMC8573821 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature