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Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds.

ABSTRACT: Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

SUBMITTER: Clough M

PROVIDER: S-EPMC8599611 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds.

Clough Mitchell M Chen Ichun Anderson IA Park Seong-Wook SW Ahrens Allison M AM Stirman Jeffrey N JN Smith Spencer L SL Chen Jerry L JL

Nature communications 20211117 1

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity acros ...[more]

PMID: 34789730

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