Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit.

ABSTRACT: Although many whole-cell biosensors (WCBs) for the detection of Cd²⁺ have been developed over the years, most lack sensitivity and specificity. In this paper, we developed a Cd²⁺ WCB with a negative feedback amplifier in P. putida KT2440. Based on the slope of the linear detection curve as a measure of sensitivity, WCB with negative feedback amplifier greatly increased the output signal of the reporter mCherry, resulting in 33% greater sensitivity than in an equivalent WCB without the negative feedback circuit. Moreover, WCB with negative feedback amplifier exhibited increased Cd²⁺ tolerance and a lower detection limit of 0.1 nM, a remarkable 400-fold improvement compared to the WCB without the negative feedback circuit, which is significantly below

SUBMITTER: Zhang G 

PROVIDER: S-EPMC8678453 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature