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Entropy-regularized deconvolution of cellular cryotransmission electron tomograms.

ABSTRACT: Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).

SUBMITTER: Croxford M

PROVIDER: S-EPMC8685678 | biostudies-literature |

REPOSITORIES: biostudies-literature

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