Reconstitution of GABA, Glycine and Glutamate Transporters.

ABSTRACT: In contrast to water soluble enzymes which can be purified and studied while in solution, studies of solute carrier (transporter) proteins require both that the protein of interest is situated in a phospholipid membrane and that this membrane forms a closed compartment. An additional challenge to the study of transporter proteins has been that the transport depends on the transmembrane electrochemical gradients. Baruch I. Kanner understood this early on and first developed techniques for studying plasma membrane vesicles. This advanced the field in that the experimenter could control the electrochemical gradients. Kanner, however, did not stop there, but started to solubilize the membranes so that the transporter proteins were taken out of their natural environment. In order to study them,

SUBMITTER: Danbolt NC 

PROVIDER: S-EPMC8763731 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature