An RNA Interference Tool to Silence Genes in Sarcoptes scabiei Eggs.

ABSTRACT: In a quest for new interventions against scabies-a highly significant skin disease of mammals, caused by a parasitic mite Sarcoptes scabiei-we are focusing on finding new intervention targets. RNA interference (RNAi) could be an efficient functional genomics approach to identify such targets. The RNAi pathway is present in S. scabiei and operational in the female adult mite, but other developmental stages have not been assessed. Identifying potential intervention targets in the egg stage is particularly important because current treatments do not kill this latter stage. Here, we established an RNAi tool to silence single-copy genes in S. scabiei eggs. Using sodium hypochlorite pre-treatment, we succeeded in rendering the eggshell permeable to dsRNA without affecting la

SUBMITTER: Fernando DD 

PROVIDER: S-EPMC8777771 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature