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Predicting bacterial promoter function and evolution from random sequences.

ABSTRACT: Predicting function from sequence is a central problem of biology. Currently, this is possible only locally in a narrow mutational neighborhood around a wildtype sequence rather than globally from any sequence. Using random mutant libraries, we developed a biophysical model that accounts for multiple features of σ⁷⁰ binding bacterial promoters to predict constitutive gene expression levels from any sequence. We experimentally and theoretically estimated that 10-20% of random sequences lead to expression and ~80% of non-expressing sequences are one mutation away from a functional promoter. The potential for generating expression from random sequences is so pervasive that selection acts against σ⁷⁰-RNA polymerase binding sites even within inter-genic, promoter-containing regions. This pervasiveness of σ⁷⁰-binding sites implies that emergence of promoters is not the limiting step in gene regulatory evolution. Ultimately, the inclusion of novel features of promoter function into a mechanistic model enabled not only more accurate predictions of gene expression levels, but also identified that promoters evolve more rapidly than previously thought.

SUBMITTER: Lagator M

PROVIDER: S-EPMC8791639 | biostudies-literature |

REPOSITORIES: biostudies-literature

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