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DgMYB2 improves cold resistance in chrysanthemum by directly targeting DgGPX1.

ABSTRACT: MYB transcription factors play important roles in plant responses to cold stress, but the associated underlying mechanisms remain unclear. In this study, a cold-induced MYB transcription factor, DgMYB2, was isolated from chrysanthemum (Chrysanthemum morifolium Ramat). DgMYB2 was localized to the nucleus and exhibited transactivational activity. Overexpression of DgMYB2 improved cold tolerance in chrysanthemum, while cold tolerance in the antisense suppression lines decreased compared to that of the wild type. Additionally, electrophoretic mobility shift assays, chromatin immunoprecipitation, luciferase complementary imaging analysis, and dual-luciferase reporter gene detection experiments confirmed that DgMYB2 directly targets DgGPX1 and increases the activity of glutathione peroxidase to reduce the accumulation of reactive oxygen species, thereby improving cold resistance in chrysanthemum.

SUBMITTER: Yang X

PROVIDER: S-EPMC8801720 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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DgMYB2 improves cold resistance in chrysanthemum by directly targeting DgGPX1.

Yang Xiaohan X Luo Yunchen Y Bai Huiru H Li Xin X Tang Si S Liao Xiaoqin X Zhang Lei L Liu Qinglin Q

Horticulture research 20220118

MYB transcription factors play important roles in plant responses to cold stress, but the associated underlying mechanisms remain unclear. In this study, a cold-induced MYB transcription factor, DgMYB2, was isolated from chrysanthemum (Chrysanthemum morifolium Ramat). DgMYB2 was localized to the nucleus and exhibited transactivational activity. Overexpression of DgMYB2 improved cold tolerance in chrysanthemum, while cold tolerance in the antisense suppression lines decreased compared to that of ...[more]

PMID: 35039835

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