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Ferrocene-Grafted Carbon Nanotubes for Sensitive Non-Enzymatic Electrochemical Detection of Hydrogen Peroxide.

ABSTRACT: Sensitive detection of hydrogen peroxide (H2O2) residue in aseptic packaging at point of use is critical to food safety. We present a sensitive non-enzymatic, amperometric H2O2 sensor based on ferrocene-functionalized multi-walled carbon nanotubes (MWCNT-FeC) and facile screen-printed carbon electrodes (SPCEs). The sensor utilizes the covalently grafted ferrocene as an effective redox mediator and the MWCNT networks to provide a large active surface area for efficient electrocatalytic reactions. The electrocatalytic MWCNT-FeC modified electrodes feature a high-efficiency electron transfer and a high electrocatalytic activity towards H2O2 reduction at a low potential of -0.15 V vs. Ag/AgCl. The decreased operating potential improves the selectivity by inherently eliminating the cross-reactivity with other electroactive interferents, such as dopamine, glucose, and ascorbic acid. The sensor exhibits a wide linear detection range from 1 μM to 1 mM with a detection limit of 0.49 μM (S/N=3). The covalently functionalized electrodes offered highly reproducible and reliable detection, providing a robust property for continuous, real-time H2O2 monitoring. Furthermore, the proposed sensor was successfully employed to determine H2O2 levels in spiked packaged milk and apple juice with satisfactory recoveries (94.33-97.62%). The MWCNT-FeC modified SPCEs offered a facile, cost-effective method for highly sensitive and selective point-of-use detection of H2O2.

SUBMITTER: Wu B

PROVIDER: S-EPMC8896809 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Ferrocene-Grafted Carbon Nanotubes for Sensitive Non-Enzymatic Electrochemical Detection of Hydrogen Peroxide.

Wu Bo B Yeasmin Sanjida S Liu Ye Y Cheng Li-Jing LJ

Journal of electroanalytical chemistry (Lausanne, Switzerland) 20220131

Sensitive detection of hydrogen peroxide (H2O2) residue in aseptic packaging at point of use is critical to food safety. We present a sensitive non-enzymatic, amperometric H2O2 sensor based on ferrocene-functionalized multi-walled carbon nanotubes (MWCNT-FeC) and facile screen-printed carbon electrodes (SPCEs). The sensor utilizes the covalently grafted ferrocene as an effective redox mediator and the MWCNT networks to provide a large active surface ar ...[more]

PMID: 35250418

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Project description:Thiol proteins are important in cellular antioxidant defenses and redox signalling. It is postulated that reactive oxidants cause selective thiol oxidation, but relative sensitivities of different cell proteins and critical targets are not well characterized. We exposed Jurkat cells to H2O2 for 10 min and measured changes in reversibly oxidized proteins by labelling with iodoacetamidofluorescein and two-dimensional electrophoresis. At 200 microM H2O2, which caused activation of the MAP (mitogen-activated protein) kinase ERK (extracellular-signal-regulated kinase), growth arrest and apoptosis, relatively few changes were seen. A total of 28 spots were reversibly oxidized (increased labelling intensity) and 24 decreased. The latter included isoforms of peroxiredoxins 1 and 2, which were irreversibly oxidized. Oxidation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was striking, and other affected proteins included glutathione S-transferase P1-1, enolase, a regulatory subunit of protein kinase A, annexin VI, the mitotic checkpoint serine/threonine-protein kinase BUB1beta, HSP90beta (heat-shock protein 90beta) and proteosome components. At 20 microM H2O2, changes were fewer, but GAPDH and peroxiredoxin 2 were still modified. Dinitrochlorobenzene treatment, which inhibited cellular thioredoxin reductase and partially depleted GSH, caused reversible oxidation of several proteins, including thioredoxin 1 and peroxiredoxins 1 and 2. Most changes were distinct from those with H2O2, and changes with H2O2 were scarcely enhanced by dinitrochlorobenzene. Relatively few proteins, including deoxycytidine kinase, nucleoside diphosphate kinase and a proteosome activator subunit, responded only to the combined treatment. Thus most of the effects of H2O2 were not linked to thioredoxin oxidation. Our study has identified peroxiredoxin 2 and GAPDH as two of the most oxidant-sensitive cell proteins and has highlighted how readily peroxiredoxins undergo irreversible oxidation.

Dataset Information

Ferrocene-Grafted Carbon Nanotubes for Sensitive Non-Enzymatic Electrochemical Detection of Hydrogen Peroxide.

Publications

Ferrocene-Grafted Carbon Nanotubes for Sensitive Non-Enzymatic Electrochemical Detection of Hydrogen Peroxide.

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