Ontology highlight
ABSTRACT:
SUBMITTER: Opoku-Nsiah KA
PROVIDER: S-EPMC8907193 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Opoku-Nsiah Kwadwo A KA de la Pena Andres H AH Williams Sarah K SK Chopra Nikita N Sali Andrej A Lander Gabriel C GC Gestwicki Jason E JE
Nature communications 20220309 1
The 20S proteasome (20S) facilitates turnover of most eukaryotic proteins. Substrate entry into the 20S first requires opening of gating loops through binding of HbYX motifs that are present at the C-termini of certain proteasome activators (PAs). The HbYX motif has been predominantly characterized in the archaeal 20S, whereas little is known about the sequence preferences of the human 20S (h20S). Here, we synthesize and screen ~120 HbYX-like peptides, revealing unexpected differences from the a ...[more]