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Synthesis of [7-¹⁵N]-GTPs for RNA structure and dynamics by NMR spectroscopy.

ABSTRACT: Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-¹⁵N]-guanosine-5'-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.

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Supplementary information

The online version contains supplementary material available at 10.1007/s00706-022-02892-1.

SUBMITTER: Taiwo KM

PROVIDER: S-EPMC8948113 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Synthesis of [7-<sup>15</sup>N]-GTPs for RNA structure and dynamics by NMR spectroscopy.

Taiwo Kehinde M KM Olenginski Lukasz T LT Nußbaumer Felix F Nam Hyeyeon H Hilber Stefan S Kreutz Christoph C Dayie T Kwaku TK

Monatshefte fur chemie 20220226 3

Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-<sup>15</sup>N]-guanosine-5'-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.<h4>Graphical abstract</h4><h4>Supplementary information</h4>The online v ...[more]

PMID: 35400760

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