Antidiabetic effect of two different Ganoderma species tested in alloxan diabetic rats.
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ABSTRACT:
SUBMITTER: Raseta M
PROVIDER: S-EPMC9050389 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
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Antidiabetic effect of two different <i>Ganoderma</i> species tested in alloxan diabetic rats.
Rašeta Milena M Popović Mira M Čapo Ivan I Stilinović Nebojša N Vukmirović Saša S Milošević Biljana B Karaman Maja M
RSC advances 20200311 17
This study was designed to define total protein, phenol and flavonoid content as well as LC-MS/MS phenolic profile related to antioxidant and antidiabetic activity of ethanolic (EtOH) and water extracts of <i>G. pfeifferi</i> and <i>G. resinaceum</i>. <i>G. resinaceum</i> water extract possessed the highest ability to scavenge DPPH˙ and O<sub>2</sub>˙<sup>-</sup>, while the EtOH extract of the same species showed better activity on NO˙ related to other extracts. The highest level of bioactive co ...[more]
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Project description:Caesalpinia bonduc has been used in herbal medicines for the treatment of a wide range of diseases from decades. The present study has explored the remedial potential and underlying mechanism of polyphenol extract of Caesalpinia bonduc in alloxanized diabetic rats. HPLC/MS analysis confirmed the presence of phenolics in considerable concentrations in Caesalpinia bonduc extract. Administration of different doses (250 and 500?mg/kg) of CPP extract to hyperglycemic rats for 8 weeks restored blood and serum glucose, insulin, glycosylated hemoglobin, leptin, amylin, and carbohydrate metabolizing enzymes level towards normal compared to alloxanized diabetic group. The effect of CPP extract on various genes such as Pdx-1, Ins-1, ngn-3, GLUT-4, and IRS-1 in insulin signaling pathway and Traf-4, Traf-6, and Mapk-8 in MAPK downstream JNK cascade was examined through qRT-PCR to access the core molecular mechanism involved in CPP-induced recovery of diabetes. Results have revealed that CPP extract reduced oxidative stress in pancreatic ? cells by restoring free radical scavenging potential, reducing the mRNA expression of Mapk-8, Traf-4, and Traf-6, and increasing the Pdx-1, Ins-1, ngn-3, GLUT-4, and IRS-1 expression ensuing regeneration of ? cells and subsequent insulin release from pancreas. The results obtained in this study recommend that CPP extract may be a promising therapeutic restorative agent in the treatment of diabetes mellitus.
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Project description:Introduction:Diabetes mellitus disorder characterized by increase in serum glucose level as a result of change in fat, protein metabolism, and carbohydrate. The aim of the present study was to investigate the effects of the aqueous and hydroalcoholic leaf extract of Thymus. schimperi on blood glucose levels. Methods:The aqueous and 80% methanol extracts of T. schimperi leaves were prepared. Swiss albino mice of either sex weighing 20-30 g were selected for the experiments. Mice that were made diabetic were divided into seven groups to study the antihyperglycemic effect of the extracts. Diabetes was induced by single intraperitonial injection of alloxan monohydrate (180 mg/kg body weight). Results:After diabetic mice were treated with an extract of solvent at doses of 250 and 500 mg/kg for 21 days, there were significant decreases in fasting blood glucose when compared to diabetic controls. The observed antidiabetic activity could be associated with the phytochemicals present in this plant extract. The extract of solvent also prevented body weight loss of diabetic when compared to diabetic mice group. It was also observed that the extracts have shown no acute toxicity at a dose of 2 g/kg. Conclusion:The aqueous and 80% methanol extracts of T. schimperi leaves have shown blood glucose level lowering effects in diabetic mice. Hence, the present study might support the traditional use of T. schimperi for diabetes mellitus treatment.
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