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An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide-ssDNA.

ABSTRACT: A novel biosensing platform was developed by integrating a new ssDNA aptamer and graphene oxide (GO) for highly sensitive and selective detection of liver cancer biomarkers (alpha-fetoprotein, AFP). The key concept of this biosensing platform is that the fluorescence of dye-modified ssDNA can be effectively quenched by GO after forming the hybrid structure of graphene oxide-ssDNA (GO-ssDNA). The AFP can selectively react with GO-ssDNA and lead to the decomposition of GO-ssDNA, which results in the recovery of fluorescence, and an increase in fluorescence intensity with the increasing concentration of AFP in the range of 0 to 300 pg mL^-1. The linear range was obtained from 1 to 150 pg mL^-1 and the detection limit was 0.909 pg mL^-1. Moreover, this biosensing platform can be applied to serum and cell imaging for the detection of AFP. The results show that the proposed biosensor has great potential application in AFP-related clinical diagnosis and research.

SUBMITTER: Wang CF

PROVIDER: S-EPMC9091683 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide-ssDNA.

Wang Chen-Feng CF Wang Zhen-Guang ZG Sun Xin-Yue XY Chen Meng-Jiao MJ Lv Yun-Kai YK

RSC advances 20181207 72

A novel biosensing platform was developed by integrating a new ssDNA aptamer and graphene oxide (GO) for highly sensitive and selective detection of liver cancer biomarkers (alpha-fetoprotein, AFP). The key concept of this biosensing platform is that the fluorescence of dye-modified ssDNA can be effectively quenched by GO after forming the hybrid structure of graphene oxide-ssDNA (GO-ssDNA). The AFP can selectively react with GO-ssDNA and lead to the decomposition of GO-ssDNA, which results in t ...[more]

PMID: 35559324

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Project description:Detecting physiological levels of neurotransmitters in biological samples can advance our understanding of brain disorders and lead to improved diagnostics and therapeutics. However, neurotransmitter sensors for real-world applications must reliably detect low concentrations of target analytes from small volume working samples. Herein, a platform for robust and ultrasensitive detection of dopamine, an essential neurotransmitter that underlies several brain disorders, based on graphene multitransistor arrays (gMTAs) functionalized with a selective DNA aptamer is presented. High-yield scalable methodologies optimized at the wafer level were employed to integrate multiple graphene transistors on small-size chips (4.5 × 4.5 mm). The multiple sensor array configuration permits independent and simultaneous replicate measurements of the same sample that produce robust average data, reducing sources of measurement variability. This procedure allowed sensitive and reproducible dopamine detection in ultra-low concentrations from small volume samples across physiological buffers and high ionic strength complex biological samples. The obtained limit-of-detection was 1 aM (10-18) with dynamic detection ranges spanning 10 orders of magnitude up to 100 µM (10-8), and a 22 mV/decade peak sensitivity in artificial cerebral spinal fluid. Dopamine detection in dopamine-depleted brain homogenates spiked with dopamine was also possible with a LOD of 1 aM, overcoming sensitivity losses typically observed in ion-sensitive sensors in complex biological samples. Furthermore, we show that our gMTAs platform can detect minimal changes in dopamine concentrations in small working volume samples (2 µL) of cerebral spinal fluid samples obtained from a mouse model of Parkinson's Disease. The platform presented in this work can lead the way to graphene-based neurotransmitter sensors suitable for real-world academic and pre-clinical pharmaceutical research as well as clinical diagnosis.

Dataset Information

An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide-ssDNA.

Publications

An ultrasensitive fluorescent aptasensor for detection of cancer marker proteins based on graphene oxide-ssDNA.

Similar Datasets

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