Browse
Submit Data
Databases
API
Help

Dataset Information

29 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mn-Containing Bioactive Glass-Ceramics: BMP-2-Mimetic Peptide Covalent Grafting Boosts Human-Osteoblast Proliferation and Mineral Deposition.

ABSTRACT: The addition of Mn in bioceramic formulation is gaining interest in the field of bone implants. Mn activates human osteoblast (h-osteoblast) integrins, enhancing cell proliferation with a dose-dependent effect, whereas Mn-enriched glasses induce inhibition of Gram-negative or Gram-positive bacteria and fungi. In an effort to further optimize Mn-containing scaffolds' beneficial interaction with h-osteoblasts, a selective and specific covalent functionalization with a bioactive peptide was carried out. The anchoring of a peptide, mapped on the BMP-2 wrist epitope, to the scaffold was performed by a reaction between an aldehyde group of the peptide and the aminic groups of silanized Mn-containing bioceramic. SEM-EDX, FT-IR, and Raman studies confirmed the presence of the peptide grafted onto the scaffold. In in vitro assays, a significant improvement in h-osteoblast proliferation, gene expression, and calcium salt deposition after 7 days was detected in the functionalized Mn-containing bioceramic compared to the controls.

SUBMITTER: Cassari L

PROVIDER: S-EPMC9267458 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Glass-Ceramics in Dentistry: A Review.

Project description:In this review, we first briefly introduce the general knowledge of glass-ceramics, including the discovery and development, the application, the microstructure, and the manufacturing of glass-ceramics. Second, the review presents a detailed description of glass-ceramics in dentistry. In this part, the history, property requirements, and manufacturing techniques of dental glass-ceramics are reviewed. The review provided a brief description of the most prevalent clinically used examples of dental glass-ceramics, namely, mica, leucite, and lithium disilicate glass-ceramics. In addition, we also introduce the newly developed ZrO2-SiO2 nanocrystalline glass-ceramics that show great potential as a new generation of dental glass-ceramics. Traditional strengthening mechanisms of glass-ceramics, including interlocking, ZrO2-reinforced, and thermal residual stress effects, are discussed. Finally, a perspective and outlook for future directions in developing new dental glass-ceramics is provided to offer inspiration to the dental materials community.

| S-EPMC7084775 | biostudies-literature

Osteoinductive ceramics as a synthetic alternative to autologous bone grafting.

Project description:Biomaterials can be endowed with biologically instructive properties by changing basic parameters such as elasticity and surface texture. However, translation from in vitro proof of concept to clinical application is largely missing. Porous calcium phosphate ceramics are used to treat small bone defects but in general do not induce stem cell differentiation, which is essential for regenerating large bone defects. Here, we prepared calcium phosphate ceramics with varying physicochemical and structural characteristics. Microporosity correlated to their propensity to stimulate osteogenic differentiation of stem cells in vitro and bone induction in vivo. Implantation in a large bone defect in sheep unequivocally demonstrated that osteoinductive ceramics are equally efficient in bone repair as autologous bone grafts. Our results provide proof of concept for the clinical application of "smart" biomaterials.

| S-EPMC2922269 | biostudies-literature

Foamed glass ceramics-an upcycled scaffold for microbial biofilm development.

Project description:Glass, a near infinitely recyclable material, can be upcycled to create new products such as foamed glass ceramics, which are essentially a synthetic pumice-like material. This material has been demonstrated to sustain preserved biofilms which have application in various fields based on the deployability of the product and the preserved microbes. Foamed glass ceramics have increased surface area compared to typical soda-lime glass cullet. This material has been explored for variety of applications including the growth, storage and transport of biofilms and microbial colonies which can be preserved and deployed later. Here, we demonstrate the ability for microbial cultures including BioTiger™, Escherichia coli K-12, Bacillus thuringiensis, and two environmental eukaryotic cells to colonize the upcycled glass products, undergo preservation, and regrow after 84 days of storage. The growth of preserved samples is correlated to the time spent incubating prior to preservation. These results demonstrate the applicability of this novel glass-biofilm combination in which various preserved microorganisms are able to be rapidly grown after storage on an upcycled glass product.

| S-EPMC9868040 | biostudies-literature

Covalent Guanosine Mimetic Inhibitors of G12C KRAS.

Project description:Ras proteins are members of a large family of GTPase enzymes that are commonly mutated in cancer where they act as dominant oncogenes. We previously developed an irreversible guanosine-derived inhibitor, SML-8-73-1, of mutant G12C RAS that forms a covalent bond with cysteine 12. Here we report exploration of the structure-activity relationships (SAR) of hydrolytically stable analogues of SML-8-73-1 as covalent G12C KRAS inhibitors. We report the discovery of difluoromethylene bisphosphonate analogues such as compound 11, which, despite exhibiting reduced efficiency as covalent G12C KRAS inhibitors, remove the liability of the hydrolytic instability of the diphosphate moiety present in SML-8-73-1 and provide the foundation for development of prodrugs to facilitate cellular uptake. The SAR and crystallographic results reaffirm the exquisite molecular recognition that exists in the diphosphate region of RAS for guanosine nucleotides which must be considered in the design of nucleotide-competitive inhibitors.

| S-EPMC5238463 | biostudies-literature

BRITER: a BMP responsive osteoblast reporter cell line.

Project description:BackgroundBMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute such screens.Methodology/principal findingsWe have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to added BMP may be further increased by depleting the endogenous BMP2 and BMP4 proteins.ConclusionAs the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specifically and promptly to exogenously added BMP2 protein, BRITER may be used effectively for chemical or molecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signaling pathway may have clinical significance.

| S-EPMC3354957 | biostudies-literature

Glass-Crystallized Luminescence Translucent Ceramics toward High-Performance Broadband NIR LEDs.

Project description:Near-infrared (NIR) phosphor-converted light-emitting diodes (pc-LEDs) are newly emergent broadband light sources for miniaturizing optical systems like spectrometers. However, traditional converters with NIR phosphors encapsulated by organic resins suffer from low external quantum efficiency (EQE), strong thermal quenching as well as low thermal conductivity, thus limiting the device efficiency and output power. Through pressureless crystallization from the designed aluminosilicate glasses, here broadband Near-infrared (NIR) emitting translucent ceramics are developed with high EQE (59.5%) and excellent thermal stability (<10% intensity loss and negligible variation of emission profile at 150 °C) to serve as all-inorganic visible-to-NIR converters. A high-performance NIR phosphor-converted light emitting diodes is further demonstrated with a record NIR photoelectric efficiency (output power) of 21.2% (62.6 mW) at 100 mA and a luminescence saturation threshold up to 184 W cm-2 . The results can substantially expand the applications of pc-LEDs, and may open up new opportunity to design efficient broadband emitting materials.

| S-EPMC8922114 | biostudies-literature

Photocatalytic hydrogen generation of monolithic porous titanium oxide-based glass-ceramics.

Project description:A large relative surface area is crucial for high catalytic activity. Monolithic catalysts are important catalytic materials because of minimal self-degradation. Regarding large surface area catalysts, the glass-ceramics (GCs) with high formability, obtained by heat-treatment of the precursor glass, are plausible candidates. This study examines the photocatalytic behaviour of porous GCs obtained after acid leaching of MgO-TiO2-P2O5 GCs. After heat-treatment, anatase TiO2 was precipitated along with other phases. The diffraction intensity ratio between anatase and other phases was the maximum for a heat-treatment temperature of 900 °C. After acid leaching of the GCs, the relative surface area decreased with increasing TiO2 fraction; the surface area was also affected by the sample morphology. H2 generation was observed from porous GCs, while GCs without etching exhibited approximately zero activity. Thus, it was demonstrated that high surface area and prevention of the reduction reaction to Ti(III) are important for tailoring monolithic photocatalytic materials.

| S-EPMC7363924 | biostudies-literature

Magnetic Glass Ceramics by Sintering of Borosilicate Glass and Inorganic Waste.

Project description:Ceramics and glass ceramics based on industrial waste have been widely recognized as competitive products for building applications; however, there is a great potential for such materials with novel functionalities. In this paper, we discuss the development of magnetic sintered glass ceramics based on two iron-rich slags, coming from non-ferrous metallurgy and recycled borosilicate glass. The substantial viscous flow of the glass led to dense products for rapid treatments at relatively low temperatures (900-1000 °C), whereas glass/slag interactions resulted in the formation of magnetite crystals, providing ferrimagnetism. Such behavior could be exploited for applying the obtained glass ceramics as induction heating plates, according to preliminary tests (showing the rapid heating of selected samples, even above 200 °C). The chemical durability and safety of the obtained glass ceramics were assessed by both leaching tests and cytotoxicity tests.

| S-EPMC5456182 | biostudies-other

Bmp Induces Osteoblast Differentiation through both Smad4 and mTORC1 Signaling.

Project description:The bone morphogenetic protein (Bmp) family of secreted molecules has been extensively studied in the context of osteoblast differentiation. However, the intracellular signaling cascades that mediate the osteoblastogenic function of Bmp have not been fully elucidated. By profiling mRNA expression in the bone marrow mesenchymal progenitor cell line ST2, we discover that BMP2 induces not only genes commonly associated with ossification and mineralization but also genes important for general protein synthesis. We define the two groups of genes as mineralization related versus protein anabolism signatures of osteoblasts. Although it induces the expression of several Wnt genes, BMP2 activates the osteogenic program largely independently of de novo Wnt secretion. Remarkably, although Smad4 is necessary for the activation of the mineralization-related genes, it is dispensable for BMP2 to induce the protein anabolism signature, which instead critically depends on the transcription factor Atf4. Upstream of Atf4, BMP2 activates mTORC1 to stimulate protein synthesis, resulting in an endoplasmic reticulum stress response mediated by Perk. Thus, Bmp signaling induces osteoblast differentiation through both Smad4- and mTORC1-dependent mechanisms.

| S-EPMC5288572 | biostudies-literature

Boosting Energy Storage Performance of Glass Ceramics via Modulating Defect Formation During Crystallization.

Project description:Along with the demand for further miniaturization of high and pulsed power devices, it becomes more and more important to realize ultrahigh recoverable energy storage density (Wrec ) with high energy storage efficiency (η) and ultrahigh discharge energy storage density (Wd ) accompanied by high power density (Pd ) in dielectrics. To date, it remains, however, a big challenge to achieve high Wrec or Wd in glass ceramics compared to other dielectric energy storage materials. Herein, a strategy of defect formation modulation is applied to form "amorphous-disordered-ordered" microstructure in BaTiO3 -based glass ceramics so as to achieve a high Wrec of 12.04 J cm-3 with a high η of 81.1% and an ultrahigh Wd of 11.98 J cm-3 with a superb Pd of 973 MW cm-3 . This work demonstrates a feasible route to obtain glass ceramics with an outstanding energy storage performance and proves the enormous potential of glass ceramics in high and pulsed power applications.

| S-EPMC10953718 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data