An innovative dual recognition aptasensor for specific detection of Staphylococcus aureus based on Au/Fe₃O₄ binary hybrid.

ABSTRACT: Pathogenic bacteria cause disease outbreaks and threaten human health, prompting the research on advanced detection assays. Herein, we developed a selective molecular imprinted aptasensor for sensitive and prompt quantitation of Staphylococcus aureus (S. aureus) bacteria. The aptasensor was constructed by immobilization of aptamer on gold nanoparticles modified magnetic nanoparticles (apt-AuNPs@ Fe₃O₄). A functional monomer (o-phenylenediamine, o-phen) was electro-polymerized on the surface of the as-synthesized nanocomposite in the presence of a template (S. aureus). After removing S. aureus, the formed imprinted sites were available to extract pathogenic bacteria from complicated matrices. The surface morphology of the as-fabricated nanocomposites was characterized

SUBMITTER: El-Wekil MM 

PROVIDER: S-EPMC9307609 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature