Proteomining-Based Elucidation of Natural Product Biosynthetic Pathways in Streptomyces.

ABSTRACT: The genus Streptomyces is known to harbor numerous biosynthetic gene clusters (BGCs) of potential utility in synthetic biology applications. However, it is often difficult to link uncharacterized BGCs with the secondary metabolites they produce. Proteomining refers to the strategy of identifying active BGCs by correlating changes in protein expression with the production of secondary metabolites of interest. In this study, we devised a shotgun proteomics-based workflow to identify active BGCs during fermentation when a variety of compounds are being produced. Mycelia harvested during the non-producing growth phase served as the background. Proteins that were differentially expressed were clustered based on the proximity of the genes in the genome to highlight active BGCs systematica

SUBMITTER: Linardi D 

PROVIDER: S-EPMC9309509 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature