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A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase.

ABSTRACT: Hepatocellular carcinoma (HCC) is associated with a high mortality rate worldwide. The therapeutic outcomes can be significantly improved if diagnosis and treatment are initiated earlier in the disease process. Recently, the carboxylesterase (CaE) activity/level in human plasma was reported to be a novel serological biomarker candidate for HCC. In this article, we fabricated a new fluorescent probe with AIE characteristics for the rapid detection of CaE with a more reliable ratiometric response mode. The TCFISE probe showed high sensitivity (LOD: 93.0 μU/mL) and selectivity toward CaE. Furthermore, the good pH stability, superior resistance against photobleaching, and low cytotoxicity highlight the high potential of the TCFISE probe for application in the monitoring of CaE activity in complex biological samples and in live cells, tissues, and animals.

SUBMITTER: Xia M

PROVIDER: S-EPMC9313056 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase.

Xia Mengting M Li Chunbin C Liu Lingxiu L He Yumao Y Li Yongdong Y Jiang Guoyu G Wang Jianguo J

Biosensors 20220703 7

Hepatocellular carcinoma (HCC) is associated with a high mortality rate worldwide. The therapeutic outcomes can be significantly improved if diagnosis and treatment are initiated earlier in the disease process. Recently, the carboxylesterase (CaE) activity/level in human plasma was reported to be a novel serological biomarker candidate for HCC. In this article, we fabricated a new fluorescent probe with AIE characteristics for the rapid detection of CaE with a more reliable ratiometric response ...[more]

PMID: 35884287

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Project description:The development of fluorescent probes derived from thiocarbonyl compounds for reactive oxygen species has been actively pursued in recent years. However, a better understanding of the optical response behaviors of thiocarbonyl compounds toward reactive oxygen species remains a challenge. Along with this, further studies to overcome the limitation of a single emission channel and aggregation-caused quenching features of thiocarbonyl-based fluorescent probes are highly desirable. Due to the important role of hypochlorite and singlet oxygen in biological processes and their common coexistence in living systems with frequent intertransformations, the design of a fluorescent probe that can recognize both hypochlorite and singlet oxygen is of great interest. Herein, a thiocarbonyl-based ratiometric fluorescent probe (Fcoum-S) for simultaneous detection of hypochlorite and singlet oxygen in aqueous solution and living cells was designed and synthesized. Upon the addition of hypochlorite in Fcoum-S solution (phosphate-buffered saline, 10 mM, pH 7.4, 10% acetonitrile), a ratiometric fluorescence response was observed via a specific hypochlorite-promoted desulfurization reaction with a good linear relationship between the ratio of fluorescence intensities at 526 and 602 nm (I 526nm/I 602nm) and the hypochlorite concentrations (a low detection limit of 0.15 μM). Furthermore, upon green light irradiation, Fcoum-S was efficiently desulfurized to its oxo analogue (Fcoum-O) by in situ generated singlet oxygen, leading to a significant change in fluorescence. Fcoum-S could work well in an aqueous medium owing to the high reactivity of the thiocarbonyl group and the aggregation-induced emission characteristics. More importantly, Fcoum-S could target mitochondria and was successfully utilized for fluorescence imaging of mitochondrial hypochlorite/singlet oxygen in live cells. This work provides a molecular design guideline for further exploring thioketone derivatives as fluorescent probes.

Dataset Information

A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase.

Publications

A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase.

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