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From Microtiter Plates to Droplets-There and Back Again.

ABSTRACT: Droplet-based microfluidic screening techniques can benefit from interfacing established microtiter plate-based screening and sample management workflows. Interfacing tools are required both for loading preconfigured microtiter-plate (MTP)-based sample collections into droplets and for dispensing the used droplets samples back into MTPs for subsequent storage or further processing. Here, we present a collection of Digital Microfluidic Pipetting Tips (DMPTs) with integrated facilities for droplet generation and manipulation together with a robotic system for its operation. This combination serves as a bidirectional sampling interface for sample transfer from wells into droplets (w2d) and vice versa droplets into wells (d2w). The DMPT were designed to fit into 96-deep-well MTPs and prepared from glass by means of microsystems technology. The aspirated samples are converted into the channel-confined droplets' sequences separated by an immiscible carrier medium. To comply with the demands of dose-response assays, up to three additional assay compound solutions can be added to the sample droplets. To enable different procedural assay protocols, four different DMPT variants were made. In this way, droplet series with gradually changing composition can be generated for, e.g., 2D screening purposes. The developed DMPT and their common fluidic connector are described here. To handle the opposite transfer d2w, a robotic transfer system was set up and is described briefly.

SUBMITTER: Henkel T

PROVIDER: S-EPMC9316479 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows.

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Evaluation of Crystal Zenith Microtiter Plates for High-Throughput Formulation Screening.

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| S-EPMC6941220 | biostudies-literature

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Adsorptive microtiter plates as solid supports in affinity purification workflows

2021-11-25 | PXD027530 | Pride

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| S-EPMC4375971 | biostudies-literature

From Visual Exploration to Storytelling and Back Again.

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Project description: Not available

| S-EPMC1895926 | biostudies-literature

Light transmission aggregometry using pre-coated microtiter plates and a Victor X5 plate reader.

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| S-EPMC5638243 | biostudies-literature

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Project description:A controversial question in cognitive neuroscience is whether comprehension of words and sentences engages brain mechanisms specific for decoding linguistic meaning or whether language comprehension occurs through more domain-general sensorimotor processes. Accumulating behavioral and neuroimaging evidence suggests a role for cortical motor and premotor areas in passive action-related language tasks, regions that are known to be involved in action execution and observation. To examine the involvement of these brain regions in language and nonlanguage tasks, we used functional magnetic resonance imaging (fMRI) on a group of 21 healthy adults. During the fMRI session, all participants 1) watched short object-related action movies, 2) looked at pictures of man-made objects, and 3) listened to and produced short sentences describing object-related actions and man-made objects. Our results are among the first to reveal, in the human brain, a functional specialization within the ventral premotor cortex (PMv) for observing actions and for observing objects, and a different organization for processing sentences describing actions and objects. These findings argue against the strongest version of the simulation theory for the processing of action-related language.

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