Developing a Method to Estimate the Downstream Metabolite Signals from Hyperpolarized [1-¹³C]Pyruvate.

ABSTRACT: Hyperpolarized carbon-13 MRI has the advantage of allowing the study of glycolytic flow in vivo or in vitro dynamically in real-time. The apparent exchange rate constant of a metabolite dynamic signal reflects the metabolite changes of a disease. Downstream metabolites can have a low signal-to-noise ratio (SNR), causing apparent exchange rate constant inconsistencies. Thus, we developed a method that estimates a more accurate metabolite signal. This method utilizes a kinetic model and background noise to estimate metabolite signals. Simulations and in vitro studies with photon-irradiated and control groups were used to evaluate the procedure. Simulated and in vitro exchange rate constants estimated using our method were compared with the raw signal values. In vitro data were also compared

SUBMITTER: Hsieh CY 

PROVIDER: S-EPMC9332172 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature