Dataset Information

Glycan shield of the ebolavirus envelope glycoprotein GP.

ABSTRACT: The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response. GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites. Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies. Here, we use glycoproteomics to profile the site-specific glycosylation patterns of ebolavirus GP. We detect up to 16 unique O-linked glycosylation sites in the MLD, and two O-linked sites in the receptor-binding GP1 subunit. Multiple O-linked glycans are observed within N-linked glycosylation sequons, suggesting crosstalk between the two types of modifications. We confirmed C-mannosylation of W288 in full-length trimeric GP. We find complex glycosylation at the majority of N-linked sites, while the conserved sites N257 and especially N563 are enriched in unprocessed glycans, suggesting a role in host-cell attachment via DC-SIGN/L-SIGN. Our findings illustrate how N-, O-, and C-linked glycans together build the heterogeneous glycan shield of GP, guiding future immunological studies and functional interpretation of ebolavirus GP-antibody interactions.

SUBMITTER: Peng W

PROVIDER: S-EPMC9352669 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Glycan shield of the ebolavirus envelope glycoprotein GP.

Peng Weiwei W Rayaprolu Vamseedhar V Parvate Amar D AD Pronker Matti F MF Hui Sean S Parekh Diptiben D Shaffer Kelly K Yu Xiaoying X Saphire Erica O EO Snijder Joost J

Communications biology 20220804 1

The envelope glycoprotein GP of the ebolaviruses is essential for host cell entry and the primary target of the host antibody response. GP is heavily glycosylated with up to 17 N-linked sites, numerous O-linked glycans in its disordered mucin-like domain (MLD), and three predicted C-linked mannosylation sites. Glycosylation is important for host cell attachment, GP stability and fusion activity, and shielding from neutralization by serum antibodies. Here, we use glycoproteomics to profile the si ...[more]

PMID: 35927436

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Dataset Information

Glycan shield of the ebolavirus envelope glycoprotein GP.

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Glycan shield of the ebolavirus envelope glycoprotein GP.

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