Dataset Information

Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects.

ABSTRACT: To disclose the net effect of light on microalgal growth in photobioreactors, self-shading and mixing-induced light-dark cycles must be minimized and discerned from the transient phenomena of acclimation. In this work, we performed experiments of continuous microalgal cultivation in small-scale photobioreactors with different thicknesses (from 2 to 35 mm): working at a steady state allowed us to describe the effect of light after acclimation, while the geometry of the reactor was adjusted to find the threshold light path that can discriminate different phenomena. Experiments showed an increased inhibition under smaller culture light paths, suggesting a strong shading effect at thicknesses higher than 8 mm where mixing-induced light-dark cycles may occur. A Haldane-like model was applied and kinetic parameters retrieved, showing possible issues in the scalability of experimental results at different light paths if mixing-induced light-dark cycles are not considered. To further highlight the influence of mixing cycles, we proposed an analogy between small-scale operations with continuous light and PBR operations with pulsed light, with the computation of characteristic parameters from pulsed-light microalgae growth mathematical modeling.

SUBMITTER: Saccardo A

PROVIDER: S-EPMC9402969 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects.

Saccardo Alberto A Bezzo Fabrizio F Sforza Eleonora E

Frontiers in bioengineering and biotechnology 20220811

To disclose the net effect of light on microalgal growth in photobioreactors, self-shading and mixing-induced light-dark cycles must be minimized and discerned from the transient phenomena of acclimation. In this work, we performed experiments of continuous microalgal cultivation in small-scale photobioreactors with different thicknesses (from 2 to 35 mm): working at a steady state allowed us to describe the effect of light after acclimation, while the geometry of the reactor was adjusted to fin ...[more]

PMID: 36032730

Similar Datasets

Project description:Overproduction of recombinant secretory proteins triggers numerous physiological perturbations. Depending on a given heterologous protein characteristics, the producer cell is faced with different challenges which lead to varying responses in terms of its physiology and the target protein production rate. In the present study, we used steady-state-maintained Yarrowia lipolytica cells to investigate the impact of different heterologous proteins on the physiological behavior of the host cells. Such an approach allowed to uncouple the impact of the overproduction of a particular protein from the phenomena that result from growth phase or are caused by the heterogeneity of the analyzed populations. Altogether, eight variants of recombinant strains, individually overproducing heterologous proteins of varying molecular weight (27-65 kDa) and reporting activity (enzymatic and fluorescent) were subjected to chemostat cultivations. The steady-state-maintained cells were analyzed in terms of the substrate utilization, biomass and metabolites production, as well as the reporter protein synthesis. Simplified distribution of carbon and nitrogen between the respective products, as well as expression analysis of the heterologous genes were conducted. The here-obtained data suggest that using a more transcriptionally active promoter results in channeling more C flux towards the target protein, giving significantly higher specific amounts and production rates of the target polypeptide, at the cost of biomass accumulation, and with no significant impact on the polyols production. The extent of the reporter protein's post-translational modifications, i.e., the number of disulfide bonds and glycosylation pattern, strongly impacts the synthesis process. Specific responses in terms of the protein formation kinetics, the gene expression levels, and transcript-to-protein linearity were observed.Key Points• Eight expression systems, producing different reporter proteins were analyzed.• The cells were maintained in steady-state by continuous chemostat culturing.• Protein- and promoter-specific effects were observed.

Dataset Information

Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects.

Publications

Microalgae growth in ultra-thin steady-state continuous photobioreactors: assessing self-shading effects.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets