Ontology highlight
ABSTRACT:
SUBMITTER: Mafireyi TJ
PROVIDER: S-EPMC9438933 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Mafireyi Tendai J TJ Laws Madeleine M Bassett John W JW Cassidy Pamela B PB Escobedo Jorge O JO Strongin Robert M RM
Angewandte Chemie (International ed. in English) 20200617 35
We report the first diselenide-based probe for the selective detection of thioredoxin reductase (TrxR), an enzyme commonly overexpressed in melanomas. The probe design involves conjugation of a seminaphthorhodafluor dye with a diselenide moiety. TrxR reduces the diselenide bond, triggering a fluorescence turn-on response of the probe. Kinetic studies reveal favorable binding of the probe with TrxR with a Michaelis-Menten constant (K<sub>m</sub> ) of 15.89 μm. Computational docking simulations pr ...[more]