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Robust neuronal differentiation of human embryonic stem cells for neurotoxicology.

ABSTRACT: Here, we describe a protocol for rapid neuronal differentiation from human embryonic stem cells (hESCs) toward a heterogenous population of telencephalic progenitors, immature and mature neurons, for drug-screening and early-brain differentiation studies. hESC neuronal differentiation depends on adhesion and minimal cell-passaging to avert monolayer cross-connectivity rupture. In this protocol, we detail optimized cell-seeding densities and coating conditions with high cell viability suitable for neurotoxicology and high-resolution single-cell omics studies. Daily media changes reduce compound instability and degradation for optimal screening. For complete details on the use and execution of this protocol, please refer to Samara et al. (2022).

SUBMITTER: Samara A

PROVIDER: S-EPMC9485591 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Robust neuronal differentiation of human embryonic stem cells for neurotoxicology.

Samara Athina A Falck Martin M Spildrejorde Mari M Leithaug Magnus M Acharya Ganesh G Lyle Robert R Eskeland Ragnhild R

STAR protocols 20220901 3

Here, we describe a protocol for rapid neuronal differentiation from human embryonic stem cells (hESCs) toward a heterogenous population of telencephalic progenitors, immature and mature neurons, for drug-screening and early-brain differentiation studies. hESC neuronal differentiation depends on adhesion and minimal cell-passaging to avert monolayer cross-connectivity rupture. In this protocol, we detail optimized cell-seeding densities and coating conditions with high cell viability suitable fo ...[more]

PMID: 36123835

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