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1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii.

ABSTRACT: To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the 1H, 13C, and 15N NMR chemical shift assignment for BeKdgF is reported.

SUBMITTER: Petersen AB

PROVIDER: S-EPMC9510102 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii.

Petersen Agnes Beenfeldt AB Christensen Idd Andrea IA Rønne Mette E ME Stender Emil G P EGP Teze David D Svensson Birte B Aachmann Finn Lillelund FL

Biomolecular NMR assignments 20220830 2

To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the 1H, 13C, and 15N NMR chem ...[more]

PMID: 36042150

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