TRNA methylation resolves codon usage bias at the limit of cell viability.
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ABSTRACT:
SUBMITTER: Masuda I
PROVIDER: S-EPMC9643105 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
Publications
tRNA methylation resolves codon usage bias at the limit of cell viability.
Masuda Isao I Yamaki Yuka Y Detroja Rajesh R Tagore Somnath S Moore Henry H Maharjan Sunita S Nakano Yuko Y Christian Thomas T Matsubara Ryuma R Lowe Todd M TM Frenkel-Morgenstern Milana M Hou Ya-Ming YM
Cell reports 20221001 4
Codon usage of each genome is closely correlated with the abundance of tRNA isoacceptors. How codon usage bias is resolved by tRNA post-transcriptional modifications is largely unknown. Here we demonstrate that the N<sup>1</sup>-methylation of guanosine at position 37 (m<sup>1</sup>G37) on the 3'-side of the anticodon, while not directly responsible for reading of codons, is a neutralizer that resolves differential decoding of proline codons. A genome-wide suppressor screen of a non-viable Esche ...[more]
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