Unknown

Dataset Information

0

Allele substitution of the streptokinase gene reduces the nephritogenic capacity of group A streptococcal strain NZ131.


ABSTRACT: To investigate the role of allelic variants of streptokinase in the pathogenesis of acute poststreptococcal glomerulonephritis (APSGN), site-specific integration plasmids were constructed, which contained either the non-nephritis-associated streptokinase gene (skc5) from the group C streptococcal strain Streptococcus equisimilis H46A or the nephritis-associated streptokinase gene (ska1) from the group A streptococcal nephritogenic strain NZ131. The plasmids were introduced by electroporation and homologous recombination into the chromosome of an isogenic derivative of strain NZ131, in which the streptokinase gene had been deleted and which had thereby lost its nephritogenic capacity in a mouse model of APSGN. The introduction of a non-nephritis-associated allelic variant of streptokinase did not rescue the nephritogenic capacity of the strain. The mutant and the wild-type strains produced equivalent amounts of streptokinase. Complementation of the ska deletion derivative with the original ska allele reconstituted the nephritogenicity of wild-type NZ131. The findings support the hypothesis that the role of streptokinase in the pathogenesis of APSGN is related to the allelic variant of the protein.

SUBMITTER: Nordstrand A 

PROVIDER: S-EPMC97243 | biostudies-literature | 2000 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Allele substitution of the streptokinase gene reduces the nephritogenic capacity of group A streptococcal strain NZ131.

Nordstrand A A   McShan W M WM   Ferretti J J JJ   Holm S E SE   Norgren M M  

Infection and immunity 20000301 3


To investigate the role of allelic variants of streptokinase in the pathogenesis of acute poststreptococcal glomerulonephritis (APSGN), site-specific integration plasmids were constructed, which contained either the non-nephritis-associated streptokinase gene (skc5) from the group C streptococcal strain Streptococcus equisimilis H46A or the nephritis-associated streptokinase gene (ska1) from the group A streptococcal nephritogenic strain NZ131. The plasmids were introduced by electroporation and  ...[more]

Similar Datasets

| S-EPMC5603603 | biostudies-literature
| S-EPMC3605901 | biostudies-literature
| S-EPMC3295313 | biostudies-literature
2022-09-30 | GSE201342 | GEO
2020-12-22 | GSE77152 | GEO
2015-01-24 | GSE65226 | GEO
| S-EPMC2725619 | biostudies-literature
| S-EPMC2583620 | biostudies-literature
2015-01-24 | E-GEOD-65226 | biostudies-arrayexpress
| S-EPMC2605554 | biostudies-literature