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Repurposing of berbamine hydrochloride to inhibit Ebola virus by targeting viral glycoprotein.

ABSTRACT: Ebola virus (EBOV) infection leads to staggeringly high mortality rate. Effective and low-cost treatments are urgently needed to control frequent EBOV outbreaks in Africa. In this study, we report that a natural compound called berbamine hydrochloride strongly inhibits EBOV replication in vitro and in vivo. Our work further showed that berbamine hydrochloride acts by directly binding to the cleaved EBOV glycoprotein (GPcl), disrupting GPcl interaction with viral receptor Niemann-Pick C1, thus blocking the fusion of viral and cellular membranes. Our data support the probability of developing anti-EBOV small molecule drugs by targeting viral GPcl. More importantly, since berbamine hydrochloride has been used in clinic to treat leukopenia, it holds great promise of being quickly repurposed as an anti-EBOV drug.

SUBMITTER: Yi D

PROVIDER: S-EPMC9764067 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Repurposing of berbamine hydrochloride to inhibit Ebola virus by targeting viral glycoprotein.

Yi Dongrong D Li Quanjie Q Wang Han H Lv Kai K Ma Ling L Wang Yujia Y Wang Jing J Zhang Yongxin Y Liu Mingliang M Li Xiaoyu X Qi Jianxun J Shi Yi Y Gao George F GF Cen Shan S

Acta pharmaceutica Sinica. B 20220525 12

Ebola virus (EBOV) infection leads to staggeringly high mortality rate. Effective and low-cost treatments are urgently needed to control frequent EBOV outbreaks in Africa. In this study, we report that a natural compound called berbamine hydrochloride strongly inhibits EBOV replication <i>in vitro</i> and <i>in vivo</i>. Our work further showed that berbamine hydrochloride acts by directly binding to the cleaved EBOV glycoprotein (GPcl), disrupting GPcl interaction with viral receptor Niemann-Pi ...[more]

PMID: 36561997

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