A hybrid system for the overproduction of complex ergot alkaloid chanoclavine.
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SUBMITTER: Ma Y
PROVIDER: S-EPMC9811125 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
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A hybrid system for the overproduction of complex ergot alkaloid chanoclavine.
Ma Yaqing Y Yan Juzhang J Yang Lujia L Yao Yongpeng Y Wang Luoyi L Gao Shu-Shan SS Cui Chengsen C
Frontiers in bioengineering and biotechnology 20221221
Synthetic biology-based methods (Sbio) and chemical synthesis (Csyn) are two independent approaches that are both widely used for synthesizing biomolecules. In the current study, two systems were combined for the overproduction of chanoclavine (CC), a structurally complex ergot alkaloid. The whole synthetic pathway for CC was split into three sections: enzymatic synthesis of 4-Br-Trp (4-Bromo-trptophan) using cell-lysate catalysis (CLC), chemical synthesis of prechanoclavine (PCC) from 4-Br-Trp, ...[more]
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