Ontology highlight
ABSTRACT:
SUBMITTER: Guardalini LGO
PROVIDER: S-EPMC9867115 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Guardalini Luis Giovani Oliveira LGO Cavalcante Paulo Eduardo da Silva PEDS Leme Jaci J Mello Renata Gois de RG Bernardino Thaissa Consoni TC Jared Simone Gonçalves Silva SGS Antoniazzi Marta Maria MM Astray Renato Mancini RM Tonso Aldo A Fernández Núñez Eutimio Gustavo EG Jorge Soraia Attie Calil SAC
Vaccines 20221224 1
This work aimed to assess, following upstream optimization in Schott flasks, the scalability from this culture platform to a stirred-tank bioreactor in order to yield rabies-recombinant baculovirus, bearing genes of G (BVG) and M (BVM) proteins, and to obtain rabies virus-like particles (VLP) from them, using Sf9 insect cells as a host. Equivalent assays in Schott flasks and a bioreactor were performed to compare both systems and a multivariate statistical approach was also carried out to maximi ...[more]