Direct cell extraction of membrane proteins for structure-function analysis.
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ABSTRACT:
SUBMITTER: Drulyte I
PROVIDER: S-EPMC9876986 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Publications
Direct cell extraction of membrane proteins for structure-function analysis.
Drulyte Ieva I Gutgsell Aspen Rene AR Lloris-Garcerá Pilar P Liss Michael M Geschwindner Stefan S Radjainia Mazdak M Frauenfeld Jens J Löving Robin R
Scientific reports 20230125 1
Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM. ...[more]
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