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Raw data for the 3D structure of horse spleen apoferritin determined by electron cryomicroscopy


ABSTRACT:

SUBMITTER: Christopher J Russo 

PROVIDER: EMPIAR-10026 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Electron microscopy: Ultrastable gold substrates for electron cryomicroscopy.

Russo Christopher J CJ   Passmore Lori A LA  

Science (New York, N.Y.) 20141201 6215


Despite recent advances, the structures of many proteins cannot be determined by electron cryomicroscopy because the individual proteins move during irradiation. This blurs the images so that they cannot be aligned with each other to calculate a three-dimensional density. Much of this movement stems from instabilities in the carbon substrates used to support frozen samples in the microscope. Here we demonstrate a gold specimen support that nearly eliminates substrate motion during irradiation. T  ...[more]

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