Ontology highlight
ABSTRACT:
SUBMITTER: Holger Stark
PROVIDER: EMPIAR-10056 | biostudies-other |
REPOSITORIES: biostudies-other
Agafonov Dmitry E DE Kastner Berthold B Dybkov Olexandr O Hofele Romina V RV Liu Wen-Ti WT Urlaub Henning H Lührmann Reinhard R Stark Holger H
Science (New York, N.Y.) 20160218 6280
The U4/U6.U5 triple small nuclear ribonucleoprotein (tri-snRNP) is a major spliceosome building block. We obtained a three-dimensional structure of the 1.8-megadalton human tri-snRNP at a resolution of 7 angstroms using single-particle cryo-electron microscopy (cryo-EM). We fit all known high-resolution structures of tri-snRNP components into the EM density map and validated them by protein cross-linking. Our model reveals how the spatial organization of Brr2 RNA helicase prevents premature U4/U ...[more]