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Structure of the human U4/U6.U5 tri-snRNP


ABSTRACT:

SUBMITTER: Holger Stark 

PROVIDER: EMPIAR-10056 | biostudies-other |

REPOSITORIES: biostudies-other

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Molecular architecture of the human U4/U6.U5 tri-snRNP.

Agafonov Dmitry E DE   Kastner Berthold B   Dybkov Olexandr O   Hofele Romina V RV   Liu Wen-Ti WT   Urlaub Henning H   Lührmann Reinhard R   Stark Holger H  

Science (New York, N.Y.) 20160218 6280


The U4/U6.U5 triple small nuclear ribonucleoprotein (tri-snRNP) is a major spliceosome building block. We obtained a three-dimensional structure of the 1.8-megadalton human tri-snRNP at a resolution of 7 angstroms using single-particle cryo-electron microscopy (cryo-EM). We fit all known high-resolution structures of tri-snRNP components into the EM density map and validated them by protein cross-linking. Our model reveals how the spatial organization of Brr2 RNA helicase prevents premature U4/U  ...[more]

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