Dataset Information
Correlative microscopy of vitreous sections provides insights into BAR-domain organisation in situ
Ontology highlight
ABSTRACT:
SUBMITTER: Wanda Kukulski
PROVIDER: EMPIAR-10161 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Correlative Microscopy of Vitreous Sections Provides Insights into BAR-Domain Organization In Situ.
Structure (London, England : 1993) 20180419 6
Electron microscopy imaging of macromolecular complexes in their native cellular context is limited by the inherent difficulty to acquire high-resolution tomographic data from thick cells and to specifically identify elusive structures within crowded cellular environments. Here, we combined cryo-fluorescence microscopy with electron cryo-tomography of vitreous sections into a coherent correlative microscopy workflow, ideal for detection and structural analysis of elusive protein assemblies in si ...[more]