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Correlative microscopy of vitreous sections provides insights into BAR-domain organisation in situ

ABSTRACT:

SUBMITTER: Wanda Kukulski

PROVIDER: EMPIAR-10161 | biostudies-other |

REPOSITORIES: biostudies-other

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Correlative Microscopy of Vitreous Sections Provides Insights into BAR-Domain Organization In Situ.

Bharat Tanmay A M TAM Hoffmann Patrick C PC Kukulski Wanda W

Structure (London, England : 1993) 20180419 6

Electron microscopy imaging of macromolecular complexes in their native cellular context is limited by the inherent difficulty to acquire high-resolution tomographic data from thick cells and to specifically identify elusive structures within crowded cellular environments. Here, we combined cryo-fluorescence microscopy with electron cryo-tomography of vitreous sections into a coherent correlative microscopy workflow, ideal for detection and structural analysis of elusive protein assemblies in si ...[more]

PMID: 29681471

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