Cryo-EM Reconstruction of apo EsCas13d
Ontology highlight
ABSTRACT:
SUBMITTER: Dmitry Lyumkis
PROVIDER: EMPIAR-10229 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d.
Zhang Cheng C Konermann Silvana S Brideau Nicholas J NJ Lotfy Peter P Wu Xuebing X Novick Scott J SJ Strutzenberg Timothy T Griffin Patrick R PR Hsu Patrick D PD Lyumkis Dmitry D
Cell 20180901 1
CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular architecture, we resolved cryoelectron microscopy structures ...[more]
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