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Tilt-schemes benchmarking for cryo electron tomography of HIV-1 CA-SP1

ABSTRACT:

SUBMITTER: Beata Turonova

PROVIDER: EMPIAR-10277 | biostudies-other |

REPOSITORIES: biostudies-other

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Benchmarking tomographic acquisition schemes for high-resolution structural biology.

Turoňová Beata B Hagen Wim J H WJH Obr Martin M Mosalaganti Shyamal S Beugelink J Wouter JW Zimmerli Christian E CE Kräusslich Hans-Georg HG Beck Martin M

Nature communications 20200213 1

Cryo electron tomography with subsequent subtomogram averaging is a powerful technique to structurally analyze macromolecular complexes in their native context. Although close to atomic resolution in principle can be obtained, it is not clear how individual experimental parameters contribute to the attainable resolution. Here, we have used immature HIV-1 lattice as a benchmarking sample to optimize the attainable resolution for subtomogram averaging. We systematically tested various experimental ...[more]

PMID: 32054835

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