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Micrographs of E. coli 70S ribosomes collected at 100 keV using a hybrid pixel direct electron detector

ABSTRACT:

SUBMITTER: Christopher John Russo

PROVIDER: EMPIAR-10316 | biostudies-other |

REPOSITORIES: biostudies-other

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CryoEM at 100 keV: a demonstration and prospects.

Naydenova K K McMullan G G Peet M J MJ Lee Y Y Edwards P C PC Chen S S Leahy E E Scotcher S S Henderson R R Russo C J CJ

IUCrJ 20191011 Pt 6

100 kV is investigated as the operating voltage for single-particle electron cryomicroscopy (cryoEM). Reducing the electron energy from the current standard of 300 or 200 keV offers both cost savings and potentially improved imaging. The latter follows from recent measurements of radiation damage to biological specimens by high-energy electrons, which show that at lower energies there is an increased amount of information available per unit damage. For frozen hydrated specimens around 300 Å in t ...[more]

PMID: 31709064

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