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Electron cryomicrographs collected as electron events on a direct electron detector for structure determination at 100 keV

ABSTRACT:

SUBMITTER: Christopher John Russo

PROVIDER: EMPIAR-11752 | biostudies-other |

REPOSITORIES: biostudies-other

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Structure determination by cryoEM at 100 keV.

McMullan Greg G Naydenova Katerina K Mihaylov Daniel D Yamashita Keitaro K Peet Mathew J MJ Wilson Hugh H Dickerson Joshua L JL Chen Shaoxia S Cannone Giuseppe G Lee Yang Y Hutchings Katherine A KA Gittins Olivia O Sobhy Mohamed A MA Wells Torquil T El-Gomati Mohamed M MM Dalby Jason J Meffert Matthias M Schulze-Briese Clemens C Henderson Richard R Russo Christopher J CJ

Proceedings of the National Academy of Sciences of the United States of America 20231127 49

Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specime ...[more]

PMID: 38011573

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