Ontology highlight
ABSTRACT:
SUBMITTER: Christopher John Russo
PROVIDER: EMPIAR-10297 | biostudies-other |
REPOSITORIES: biostudies-other
Naydenova K K McMullan G G Peet M J MJ Lee Y Y Edwards P C PC Chen S S Leahy E E Scotcher S S Henderson R R Russo C J CJ
IUCrJ 20191011 Pt 6
100 kV is investigated as the operating voltage for single-particle electron cryomicroscopy (cryoEM). Reducing the electron energy from the current standard of 300 or 200 keV offers both cost savings and potentially improved imaging. The latter follows from recent measurements of radiation damage to biological specimens by high-energy electrons, which show that at lower energies there is an increased amount of information available per unit damage. For frozen hydrated specimens around 300 Å in t ...[more]