Dataset Information
Single particle cryo-EM dataset for LetB from E.coli
Ontology highlight
ABSTRACT:
SUBMITTER: Nicolas Coudray
PROVIDER: EMPIAR-10350 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope.
Cell 20200401 3
Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have not been well characterized. Our ~3.5 Å cryo-EM structure of the E. coli MCE protein LetB reveals an ~0.6 megadalton complex that consists of seven stacked rings, with a central hydrophobic tunnel su ...[more]