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Single particle cryo-EM dataset for LetB from E.coli

ABSTRACT:

SUBMITTER: Nicolas Coudray

PROVIDER: EMPIAR-10350 | biostudies-other |

REPOSITORIES: biostudies-other

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LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope.

Isom Georgia L GL Coudray Nicolas N MacRae Mark R MR McManus Collin T CT Ekiert Damian C DC Bhabha Gira G

Cell 20200401 3

Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have not been well characterized. Our ~3.5 Å cryo-EM structure of the E. coli MCE protein LetB reveals an ~0.6 megadalton complex that consists of seven stacked rings, with a central hydrophobic tunnel su ...[more]

PMID: 32359438

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