Dataset Information
Frame aligned tilt series of cryo-FIB milled HEK293 cells expressing pathogenic LRRK2(I2020T)mutant
Ontology highlight
ABSTRACT:
SUBMITTER: Elizabeth Villa
PROVIDER: EMPIAR-10378 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
The In Situ Structure of Parkinson's Disease-Linked LRRK2.
Cell 20200811 6
Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent cause of familial Parkinson's disease. LRRK2 is a multi-domain protein containing a kinase and GTPase. Using correlative light and electron microscopy, in situ cryo-electron tomography, and subtomogram analysis, we reveal a 14-Å structure of LRRK2 bearing a pathogenic mutation that oligomerizes as a right-handed double helix around microtubules, which are left-handed. Using integrative modeling, we determine the architecture ...[more]