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Single-particle cryo-EM of the human CDK-activating kinase in complex with ATP-gamma-S

ABSTRACT:

SUBMITTER: Basil J Greber

PROVIDER: EMPIAR-10431 | biostudies-other |

REPOSITORIES: biostudies-other

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The cryoelectron microscopy structure of the human CDK-activating kinase.

Greber Basil J BJ Perez-Bertoldi Juan M JM Lim Kif K Iavarone Anthony T AT Toso Daniel B DB Nogales Eva E

Proceedings of the National Academy of Sciences of the United States of America 20200827 37

The human CDK-activating kinase (CAK), a complex composed of cyclin-dependent kinase (CDK) 7, cyclin H, and MAT1, is a critical regulator of transcription initiation and the cell cycle. It acts by phosphorylating the C-terminal heptapeptide repeat domain of the RNA polymerase II (Pol II) subunit RPB1, which is an important regulatory event in transcription initiation by Pol II, and it phosphorylates the regulatory T-loop of CDKs that control cell cycle progression. Here, we have determined the t ...[more]

PMID: 32855301

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