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Single-particle Cryo-EM of the endogenous human BAF complex alone

ABSTRACT:

SUBMITTER: Thomas Walz

PROVIDER: EMPIAR-10590 | biostudies-other |

REPOSITORIES: biostudies-other

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A Structural Model of the Endogenous Human BAF Complex Informs Disease Mechanisms.

Mashtalir Nazar N Suzuki Hiroshi H Farrell Daniel P DP Sankar Akshay A Luo Jie J Filipovski Martin M D'Avino Andrew R AR St Pierre Roodolph R Valencia Alfredo M AM Onikubo Takashi T Roeder Robert G RG Han Yan Y He Yuan Y Ranish Jeffrey A JA DiMaio Frank F Walz Thomas T Kadoch Cigall C

Cell 20201013 3

Mammalian SWI/SNF complexes are ATP-dependent chromatin remodeling complexes that regulate genomic architecture. Here, we present a structural model of the endogenously purified human canonical BAF complex bound to the nucleosome, generated using cryoelectron microscopy (cryo-EM), cross-linking mass spectrometry, and homology modeling. BAF complexes bilaterally engage the nucleosome H2A/H2B acidic patch regions through the SMARCB1 C-terminal α-helix and the SMARCA4/2 C-terminal SnAc/post-SnAc re ...[more]

PMID: 33053319

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