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DNA origami signposts for identifying proteins on cell membranes by electron cryotomography


ABSTRACT:

SUBMITTER: Lindsay A Baker 

PROVIDER: EMPIAR-10613 | biostudies-other |

REPOSITORIES: biostudies-other

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Electron cryotomography (cryoET), an electron cryomicroscopy (cryoEM) modality, has changed our understanding of biological function by revealing the native molecular details of membranes, viruses, and cells. However, identification of individual molecules within tomograms from cryoET is challenging because of sample crowding and low signal-to-noise ratios. Here, we present a tagging strategy for cryoET that precisely identifies individual protein complexes in tomograms without relying on metal  ...[more]

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