DNA origami signposts for identifying proteins on cell membranes by electron cryotomography
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ABSTRACT:
SUBMITTER: Lindsay A Baker
PROVIDER: EMPIAR-10613 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.
Silvester Emma E Vollmer Benjamin B Pražák Vojtěch V Vasishtan Daven D Machala Emily A EA Whittle Catheryne C Black Susan S Bath Jonathan J Turberfield Andrew J AJ Grünewald Kay K Baker Lindsay A LA
Cell 20210201 4
Electron cryotomography (cryoET), an electron cryomicroscopy (cryoEM) modality, has changed our understanding of biological function by revealing the native molecular details of membranes, viruses, and cells. However, identification of individual molecules within tomograms from cryoET is challenging because of sample crowding and low signal-to-noise ratios. Here, we present a tagging strategy for cryoET that precisely identifies individual protein complexes in tomograms without relying on metal ...[more]
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