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CryoET dataset of T20S proteasome for testing motion-aware tilt-series alignment and 3D reconstruction

ABSTRACT:

SUBMITTER: JJ Fernandez

PROVIDER: EMPIAR-10651 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion.

Fernandez Jose-Jesus JJ Li Sam S Bharat Tanmay A M TAM Agard David A DA

Journal of structural biology 20180203 3

Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal tilt-series alignment and thus deterioration of the reconstruction quality. Here we present a novel approach to tilt-series alignment and tomographic reconstruction that considers the beam-induced sample motion through the tilt-series. It extends the standard fiducial-based alignment approach in cryoET ...[more]

PMID: 29410148

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Automated tilt series alignment and tomographic reconstruction in IMOD.

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| S-EPMC5247408 | biostudies-literature

CryoET of T20S proteasome single particle