CryoET tilt series of mouse sperm flagella after FIB-SEM milling
Ontology highlight
ABSTRACT:
SUBMITTER: Zhen Chen
PROVIDER: EMPIAR-11221 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes.
Chen Zhen Z Greenan Garrett A GA Shiozaki Momoko M Liu Yanxin Y Skinner Will M WM Zhao Xiaowei X Zhao Shumei S Yan Rui R Yu Zhiheng Z Lishko Polina V PV Agard David A DA Vale Ronald D RD
Nature structural & molecular biology 20230102 3
The flagella of mammalian sperm display non-planar, asymmetric beating, in contrast to the planar, symmetric beating of flagella from sea urchin sperm and unicellular organisms. The molecular basis of this difference is unclear. Here, we perform in situ cryo-electron tomography of mouse and human sperm, providing the highest-resolution structural information to date. Our subtomogram averages reveal mammalian sperm-specific protein complexes within the microtubules, the radial spokes and nexin-dy ...[more]
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