Structural mechanism of GTPase-powered ribosome-tRNA movement
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ABSTRACT:
SUBMITTER: Niels Fischer
PROVIDER: EMPIAR-10792 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural mechanism of GTPase-powered ribosome-tRNA movement.
Petrychenko Valentyn V Peng Bee-Zen BZ de A P Schwarzer Ana C AC Peske Frank F Rodnina Marina V MV Fischer Niels N
Nature communications 20211011 1
GTPases are regulators of cell signaling acting as molecular switches. The translational GTPase EF-G stands out, as it uses GTP hydrolysis to generate force and promote the movement of the ribosome along the mRNA. The key unresolved question is how GTP hydrolysis drives molecular movement. Here, we visualize the GTPase-powered step of ongoing translocation by time-resolved cryo-EM. EF-G in the active GDP-Pi form stabilizes the rotated conformation of ribosomal subunits and induces twisting of th ...[more]
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