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Cryo electron tomograms of mouse DRG axons (dataset 2)

ABSTRACT:

SUBMITTER: Helen Elizabeth Foster

PROVIDER: EMPIAR-10814 | biostudies-other |

REPOSITORIES: biostudies-other

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A cryo-ET survey of microtubules and intracellular compartments in mammalian axons.

Foster Helen E HE Ventura Santos Camilla C Carter Andrew P AP

The Journal of cell biology 20211208 2

The neuronal axon is packed with cytoskeletal filaments, membranes, and organelles, many of which move between the cell body and axon tip. Here, we used cryo-electron tomography to survey the internal components of mammalian sensory axons. We determined the polarity of the axonal microtubules (MTs) by combining subtomogram classification and visual inspection, finding MT plus and minus ends are structurally similar. Subtomogram averaging of globular densities in the MT lumen suggests they have a ...[more]

PMID: 34878519

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